Cyclodextrin glycosyltransferase biosynthesis improvement by recombinantLactococcus lactisNZ:NSP:CGT: medium formulation and culture condition optimization
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منابع مشابه
Integral Use of Amaranth Starch to Obtain Cyclodextrin Glycosyltransferase, by Bacillus megaterium, to Produce β-Cyclodextrin
Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrins (CDs) from starch and related carbohydrates, producing a mixture of α-, β-, and γ-CDs in different amounts. CGTase production, mainly by Bacillus sp., depends on fermentation conditions such as pH, temperature, concentration of nutrients, carbon and nitrogen sources, among others. Bacillus megaterium CGTase produ...
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Streptomyces sp. JAJ06 is a seawater-dependent antibiotic producer, previously isolated and characterised from an Indian coastal solar saltern. This paper reports replacement of seawater with a defined salt formulation in production medium and subsequent statistical media optimization to ensure consistent as well as improved antibiotic production by Streptomyces sp. JAJ06. This strain was obser...
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The cyclodextrin glycosyltransferase (CGTase) of Paenibacillus pabuli US132 was fused to the secretive lipase signal peptide of B. subtilis. This leads to an efficient secretion of the recombinant enzyme into the culture medium of E. coli as an active and soluble form contrasting with the native construction leading to a periplasmic production. In order to enhance the yield of CGTase production...
متن کاملCyclodextrin Formation by the Thermostable a-Amylase of Thermoanaerobacterium thermosulfurigenes EM1 and Reclassification of the Enzyme as a Cyclodextrin Glycosyltransferase
glycosyltransferase. the enzyme as a cyclodextrin thermosulfurigenes EM1 and reclassification of alpha-amylase of Thermoanaerobacterium Cyclodextrin formation by the thermostable
متن کاملCyclodextrin formation by the thermostable alpha-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase.
Extensive characterization of the thermostable alpha-amylase of Clostridium thermosulfurogenes EM1, recently reclassified as Thermoanaerobacterium thermosulfurigenes, clearly demonstrated that the enzyme is a cyclodextrin glycosyltransferase (CGTase). Product analysis after incubation of the enzyme with starch revealed formation of alpha-, beta-, and gamma-cyclodextrins, as well as linear sugar...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2015
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2015.1009713